C18zip -

Repeat using the same elution volume to ensure all peptides are recovered.

Repeat . This prepares the resin to bind the peptides. 4. Sample Binding

The sample is now ready for (MALDI-TOF or LC-MS/MS). C18zip

Repeat this cycle to maximize binding to the C18 media. Note: Slow, consistent pipetting is key for high recovery. 5. Washing (Desalting) Aspirate the Wash Solution (0.1% TFA). Dispense to waste. Repeat 3 times to remove salts, buffers, and detergents. 6. Elution Aspirate the Elution Solution (e.g., 60% ACN/0.1% TFA).

Aspirate and dispense your peptide sample through the ZipTip. Repeat using the same elution volume to ensure

Below is the standard protocol for peptide desalting and concentration using C18 ZipTips. 🧪 C18 ZipTip Standard Protocol 1. Preparation & Materials

💡 If your sample contains high concentrations of salts or urea, increase the number of wash steps in Section 5 to avoid interference during mass spectrometry. Note: Slow, consistent pipetting is key for high recovery

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