: FinchTV (Windows/Mac) and Chromas Lite (Windows) are popular for basic viewing and manual base-call editing.
: If you are a bioinformatician, tools like the sangeranalyseR package in R can process an entire directory of AB1 files from your unzipped archive at once.
: Always store the original .ab1 files. While .seq files are easier to copy/paste, the .ab1 file is essential for verifying any ambiguous base calls.
: Services like the EditCo ICE tool allow you to drag and drop your .zip archive directly for CRISPR knockout or knock-in analysis. 3. Key Analysis Tips
: Select the specific run or order. Look for a link labeled "Download All Results" or "Download .zip" . This package usually includes the .ab1 trace files, .seq text files (FASTA format), and sometimes a .pdf report. 2. Opening and Viewing the Data
Inference of CRISPR Edits (ICE) - Knockout Analysis - EditCo Bio
: Go to your Order History or Data Downloads section.
: Look for high, distinct peaks. Overlapping peaks (noise) or "flat" lines usually indicate a failed sequencing reaction or poor template quality.